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Updated to magento 2.4.5 now nginx fails to start

Just upgraded to Magento 2.4.5 on Ubuntu 20.4 running nginx with a Hestia control panel.

After a reboot of the server, nginx failed to restart.

Running command sudo nginx -t brings up error:

nginx: [emerg] "root" directive is duplicate in /home/--/web/--/public_html/nginx.conf.sample:32 nginx: configuration file /etc/nginx/nginx.conf test failed

If I open nginx.conf.sample and comment out line 32, I’m faced with a similar error

nginx: [emerg] "autoindex" directive is duplicate in /home/--/web/--/public_html/nginx.conf.sample:35 nginx: configuration file /etc/nginx/nginx.conf test failed

and so on right down the file, and nginx never passes the test.

Can somebody point me in the right direction please?

Here is the entire nginx.conf.sample file – it matches the file in the Magento git.

## Example configuration:
# upstream fastcgi_backend {
#    # use tcp connection
#    # server  127.0.0.1:9000;
#    # or socket
#    server   unix:/var/run/php/php7.4-fpm.sock;
# }
# server {
#    listen 80;
#    server_name mage.dev;
#    set $MAGE_ROOT /var/www/magento2;
#    set $MAGE_DEBUG_SHOW_ARGS 0;
#    include /vagrant/magento2/nginx.conf.sample;
# }
#
## Optional override of deployment mode. We recommend you use the
## command 'bin/magento deploy:mode:set' to switch modes instead.
##
## set $MAGE_MODE default; # or production or developer
##
## If you set MAGE_MODE in server config, you must pass the variable into the
## PHP entry point blocks, which are indicated below. You can pass
## it in using:
##
## fastcgi_param  MAGE_MODE $MAGE_MODE;
##
## In production mode, you should uncomment the 'expires' directive in the /static/ location block

# Modules can be loaded only at the very beginning of the Nginx config file, please move the line below to the main config file
# load_module /etc/nginx/modules/ngx_http_image_filter_module.so;

root $MAGE_ROOT/pub;

index index.php;
autoindex off;
charset UTF-8;
error_page 404 403 = /errors/404.php;
#add_header "X-UA-Compatible" "IE=Edge";


# Deny access to sensitive files
location /.user.ini {
    deny all;
}

# PHP entry point for setup application
location ~* ^/setup($|/) {
    root $MAGE_ROOT;
    location ~ ^/setup/index.php {
        fastcgi_pass   fastcgi_backend;

        fastcgi_param  PHP_FLAG  "session.auto_start=off n suhosin.session.cryptua=off";
        fastcgi_param  PHP_VALUE "memory_limit=756M n max_execution_time=600";
        fastcgi_read_timeout 600s;
        fastcgi_connect_timeout 600s;

        fastcgi_index  index.php;
        fastcgi_param  SCRIPT_FILENAME  $document_root$fastcgi_script_name;
        include        fastcgi_params;
    }

    location ~ ^/setup/(?!pub/). {
        deny all;
    }

    location ~ ^/setup/pub/ {
        add_header X-Frame-Options "SAMEORIGIN";
    }
}

# PHP entry point for update application
location ~* ^/update($|/) {
    root $MAGE_ROOT;

    location ~ ^/update/index.php {
        fastcgi_split_path_info ^(/update/index.php)(/.+)$;
        fastcgi_pass   fastcgi_backend;
        fastcgi_index  index.php;
        fastcgi_param  SCRIPT_FILENAME  $document_root$fastcgi_script_name;
        fastcgi_param  PATH_INFO        $fastcgi_path_info;
        include        fastcgi_params;
    }

    # Deny everything but index.php
    location ~ ^/update/(?!pub/). {
        deny all;
    }

    location ~ ^/update/pub/ {
        add_header X-Frame-Options "SAMEORIGIN";
    }
}

location / {
    try_files $uri $uri/ /index.php$is_args$args;
}

location /pub/ {
    location ~ ^/pub/media/(downloadable|customer|import|custom_options|theme_customization/.*.xml) {
        deny all;
    }
    alias $MAGE_ROOT/pub/;
    add_header X-Frame-Options "SAMEORIGIN";
}

location /static/ {
    # Uncomment the following line in production mode
    # expires max;

    # Remove signature of the static files that is used to overcome the browser cache
    location ~ ^/static/versiond*/ {
        rewrite ^/static/versiond*/(.*)$ /static/$1 last;
    }

    location ~* .(ico|jpg|jpeg|png|gif|svg|svgz|webp|avif|avifs|js|css|eot|ttf|otf|woff|woff2|html|json|webmanifest)$ {
        add_header Cache-Control "public";
        add_header X-Frame-Options "SAMEORIGIN";
        expires +1y;

        if (!-f $request_filename) {
            rewrite ^/static/(versiond*/)?(.*)$ /static.php?resource=$2 last;
        }
    }
    location ~* .(zip|gz|gzip|bz2|csv|xml)$ {
        add_header Cache-Control "no-store";
        add_header X-Frame-Options "SAMEORIGIN";
        expires    off;

        if (!-f $request_filename) {
           rewrite ^/static/(versiond*/)?(.*)$ /static.php?resource=$2 last;
        }
    }
    if (!-f $request_filename) {
        rewrite ^/static/(versiond*/)?(.*)$ /static.php?resource=$2 last;
    }
    add_header X-Frame-Options "SAMEORIGIN";
}

location /media/ {

## The following section allows to offload image resizing from Magento instance to the Nginx.
## Catalog image URL format should be set accordingly.
## See https://docs.magento.com/user-guide/configuration/general/web.html#url-options
#   location ~* ^/media/catalog/.* {
#
#       # Replace placeholders and uncomment the line below to serve product images from public S3
#       # See examples of S3 authentication at https://github.com/anomalizer/ngx_aws_auth
#       # resolver 8.8.8.8;
#       # proxy_pass https://<bucket-name>.<region-name>.amazonaws.com;
#
#       set $width "-";
#       set $height "-";
#       if ($arg_width != '') {
#           set $width $arg_width;
#       }
#       if ($arg_height != '') {
#           set $height $arg_height;
#       }
#       image_filter resize $width $height;
#       image_filter_jpeg_quality 90;
#   }

    try_files $uri $uri/ /get.php$is_args$args;

    location ~ ^/media/theme_customization/.*.xml {
        deny all;
    }

    location ~* .(ico|jpg|jpeg|png|gif|svg|svgz|webp|avif|avifs|js|css|eot|ttf|otf|woff|woff2)$ {
        add_header Cache-Control "public";
        add_header X-Frame-Options "SAMEORIGIN";
        expires +1y;
        try_files $uri $uri/ /get.php$is_args$args;
    }
    location ~* .(zip|gz|gzip|bz2|csv|xml)$ {
        add_header Cache-Control "no-store";
        add_header X-Frame-Options "SAMEORIGIN";
        expires    off;
        try_files $uri $uri/ /get.php$is_args$args;
    }
    add_header X-Frame-Options "SAMEORIGIN";
}

location /media/customer/ {
    deny all;
}

location /media/downloadable/ {
    deny all;
}

location /media/import/ {
    deny all;
}

location /media/custom_options/ {
    deny all;
}

location /errors/ {
    location ~* .xml$ {
        deny all;
    }
}

# PHP entry point for main application
location ~ ^/(index|get|static|errors/report|errors/404|errors/503|health_check).php$ {
    try_files $uri =404;
    fastcgi_pass   fastcgi_backend;
    fastcgi_buffers 16 16k;
    fastcgi_buffer_size 32k;

    fastcgi_param  PHP_FLAG  "session.auto_start=off n suhosin.session.cryptua=off";
    fastcgi_param  PHP_VALUE "memory_limit=756M n max_execution_time=18000";
    fastcgi_read_timeout 600s;
    fastcgi_connect_timeout 600s;

    fastcgi_index  index.php;
    fastcgi_param  SCRIPT_FILENAME  $document_root$fastcgi_script_name;
    include        fastcgi_params;
}

gzip on;
gzip_disable "msie6";

gzip_comp_level 6;
gzip_min_length 1100;
gzip_buffers 16 8k;
gzip_proxied any;
gzip_types
    text/plain
    text/css
    text/js
    text/xml
    text/javascript
    application/javascript
    application/x-javascript
    application/json
    application/xml
    application/xml+rss
    image/svg+xml;
gzip_vary on;

# Banned locations (only reached if the earlier PHP entry point regexes don't match)
location ~* (.php$|.phtml$|.htaccess$|.htpasswd$|.git) {
    deny all;
}

I have commented out the offending line, and another line pops up with the same error, until eventually I run out of lines to comment out and I get this error instead:

nginx: [emerg] no port in upstream "fastcgi_backend" in /home/--/web/--/public_html/nginx.conf.sample:51 nginx: configuration file /etc/nginx/nginx.conf test failed

I’m now at an absolute loss. Thanks for any help you could give me.